Dot-blot assays and their use as a direct antigen-binding method to screen monoclonal antibodies to 1,4-β-and 1,3-β-glucan synthases

Abstract : A rapid method has been developed to assay β-glucan synthases spotted on a nitrocellulose sheet. The sensitivity of this method allows screening of hybridoma-making monoclonal antibodies in a direct antigen-binding assay by measurement of the activity of the enzymes retained by the antibodies previously fixed on nitrocellulose.
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Submitted on : Tuesday, February 16, 2016 - 10:55:23 AM
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Patrice Nodet, Jacques Grange, Michel Fèvre. Dot-blot assays and their use as a direct antigen-binding method to screen monoclonal antibodies to 1,4-β-and 1,3-β-glucan synthases. Analytical Biochemistry, Elsevier Masson, 1988, 174 (2), pp.662-665. ⟨10.1016/0003-2697(88)90070-X⟩. ⟨hal-01274724⟩

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