Efficient anaerobic transformation of raw wheat straw by a robust cow rumen-derived microbial consortium

Adèle Lazuka (1) , Lucas Auer (1) , Sophie Bozonnet (1) , Diego Morgavi (2) , Michael O'Donohue (1) , Guillermina Hernandez Raquet (1)

1 LISBP - Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2 UMRH - Unité Mixte de Recherche sur les Herbivores - UMR 1213

A rumen-derived microbial consortium was enriched on raw wheat straw as sole carbon source in a sequential batch-reactor (SBR) process under strict mesophilic anaerobic conditions. After five cycles of enrichment the procedure enabled to select a stable and efficient lignocellulolytic microbial consortium, mainly constituted by members of Firmicutes and Bacteroidetes phyla. The enriched community, designed rumen-wheat straw-derived consortium (RWS) efficiently hydrolyzed lignocellulosic biomass, degrading 55.5% w/w of raw wheat straw over 15 days at 35 degrees C and accumulating carboxylates as main products. Cellulolytic and hemicellulolytic activities, mainly detected on the cell bound fraction, were produced in the earlier steps of degradation, their production being correlated with the maximal lignocellulose degradation rates. Overall, these results demonstrate the potential of RWS to convert unpretreated lignocellulosic substrates into useful chemicals.

Mots clés: lignocellulose bioconversion; anaerobic consortium; carboxylate production; xylanase; cellulase

Domaines: Biotechnologies

Adèle Lazuka, Lucas Auer, Sophie Bozonnet, Diego Morgavi, Michael O'Donohue, et al.. Efficient anaerobic transformation of raw wheat straw by a robust cow rumen-derived microbial consortium. Bioresource Technology, 2015, 196, pp.241-249. ⟨10.1016/j.biortech.2015.07.084⟩. ⟨hal-01269314⟩