Skip to Main content Skip to Navigation
Journal articles

Aga1, the first alpha-Galactosidase from the human bacteria Ruminococcus gnavus E1, efficiently transcribed in gut conditions

Abstract : Differential gene expression analysis was performed in monoxenic mice colonized with Ruminococcus gnavus strain E1, a major endogenous member of the gut microbiota. RNA arbitrarily primed-PCR fingerprinting assays allowed to specifically detect the invivo expression of the aga1 gene, which was further confirmed by RT-PCR. The aga1 gene encoded a protein of 744 residues with calculated molecular mass of 85,207Da. Aga1 exhibited significant similarity with previously characterized alpha-Galactosidases of the GH 36 family. Purified recombinant protein demonstrated high catalytic activity (1047Umg(-1)) and efficient p-nitrophenyl-alpha-d-galactopyranoside hydrolysis [k(cat)/K(m)=35.1158.82s(-1)mM(-1) at 55degreesC and k(cat)/K(m)=17.484.25s(-1)mM(-1) at 37degreesC].
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-01267759
Contributor : Archive Ouverte Prodinra <>
Submitted on : Thursday, February 4, 2016 - 8:19:46 PM
Last modification on : Saturday, October 3, 2020 - 3:17:19 AM

Identifiers

Collections

Citation

Margarita Aguilera, Harivony Rakotoarivonina, Alexandre Brutus, Thierry Giardina, Gwenola Simon, et al.. Aga1, the first alpha-Galactosidase from the human bacteria Ruminococcus gnavus E1, efficiently transcribed in gut conditions. Research in Microbiology, Elsevier, 2012, 163 (1), pp.14-21. ⟨10.1016/j.resmic.2011.10.005⟩. ⟨hal-01267759⟩

Share

Metrics

Record views

544