Development of Exon-Primed Intron-Crossing (EPIC) PCR primers for the malaria vector Anopheles pseudopunctipennis (Diptera : Culicidae)

Frédéric Lardeux (1) , Claudia Aliaga (1) , Rosenka Tejerina (1) , Raul Ursic-Bedoya (2)

1 Institut de Recherche pour le Développement - IRD Bolivie
2 Department of Biological Sciences [Burnaby]

Using the Anopheles gambiae Giles genome as a template, we designed, screened and identified 14 novel Exon-Primed Intron-Crossing (EPIC) PCR primer pairs for Anopheles pseudopunctipennis Theobald 1901, a major vector of human Plasmodium sp. in South America. These primers were designed to target the conserved regions flanking consecutive exons of different genes and enabled the amplification of 17 loci of which nine were polymorphic. Polymorphisms at these loci ranged from two to four alleles. Intron length polymorphism analysis is a useful tool, which will allow the study of the population structure of this mosquito species, which remains poorly understood.

Mots clés: EPIC-PCR; Anopheles pseudopunctipennis; Malaria; Primer; Intron

Domaines: Biologie moléculaire; Génétique des populations [q-bio.PE]; Zoologie des invertébrés; Ecosystèmes; Santé publique et épidémiologie

Frédéric Lardeux, Claudia Aliaga, Rosenka Tejerina, Raul Ursic-Bedoya. Development of Exon-Primed Intron-Crossing (EPIC) PCR primers for the malaria vector Anopheles pseudopunctipennis (Diptera : Culicidae). Comptes Rendus Biologies, 2012, 335 (6), pp.398-405. ⟨10.1016/j.crvi.2012.05.002⟩. ⟨hal-01254144⟩