Determination of endocrine disruptors and endogenic androgens and estrogens in rat serum by high-performance liquid chromatography–tandem mass spectrometry

Abstract : To simultaneously measure some targeted endocrine disruptors and several forms of sex hormones in rat serum, an accurate analytical procedure was developed. First, a comparison between a polymeric-based solid-phase extraction (SPE) and a micro-extraction by packed sorbent was performed to choose the optimal method to extract and concentrate the analytes: bisphenol A, atrazine, vinclozolin metabolite, testosterone, androstenedione, estrone, estradiol, estrone-sulfate and glucuronide and estradiol-sulfate and glucuronide. The analyses were then performed by high-performance liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) with electrospray ionisation in positive and negative modes. The protocol based on SPE was validated using the ICH/2005 guidelines. The validation demonstrated good performance in terms of linearity (R2 > 0.99), recovery (71–90%) and repeatability (relative standard deviation: 1–18%). The method was sensitive with LOQ comprised between 0.1 and 0.4 ng/ml for androgens and between 0.098 and 10.2 ng/ml for estrogens. The results obtained on the serum of rats exposed to the targeted endocrine disruptors showed the suitability of this analytical strategy.
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Submitted on : Thursday, December 10, 2015 - 5:27:50 PM
Last modification on : Wednesday, August 29, 2018 - 1:01:48 PM

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Mikaël Tournier, Charlène Pouech, Nadia Quignot, Florent Lafay, Laure Wiest, et al.. Determination of endocrine disruptors and endogenic androgens and estrogens in rat serum by high-performance liquid chromatography–tandem mass spectrometry. Steroids, Elsevier, 2015, 104, pp.252-262. ⟨10.1016/j.steroids.2015.10.011⟩. ⟨hal-01241662⟩

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