Proteomic Approach to Identify Nuclear Proteins in Wheat Grain

Emmanuelle Bancel (1) , Titouan Bonnot (1) , Marlène Davanture (2) , Emmanuel Branlard (1) , Michel Zivy (2) , Pierre Martres (1)

1 GDEC - Génétique Diversité et Ecophysiologie des Céréales
2 GQE-Le Moulon - Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale)

The nuclear proteome of the grain of the two cultivated wheat species Triticum aestivum (hexaploid wheat; genomes A, B, and D) and T. monococcum (diploid wheat; genome A) was analyzed in two early stages of development using shotgun-based proteomics. A procedure was optimized to purify nuclei, and an improved protein sample preparation was developed to efficiently remove nonprotein substances (starch and nucleic acids). A total of 797 proteins corresponding to 528 unique proteins were identified, 36% of which were classified in functional groups related to DNA and RNA metabolism. A large number (107 proteins) of unknown functions and hypothetical proteins were also found. Some identified proteins may be multifunctional and may present multiple localizations. On the basis of the MS/MS analysis, 368 proteins were present in the two species, and in two stages of development, some qualitative differences between species and stages of development were also found. All of these data illustrate the dynamic function of the grain nucleus in the early stages of development.

Mots clés: LC−MS/MS; bread wheat (Triticum aestivum); cereal; einkorn wheat (Triticum monococcum); grain development; nuclear proteome

Domaines: Biologie végétale

Emmanuelle Bancel, Titouan Bonnot, Marlène Davanture, Emmanuel Branlard, Michel Zivy, et al.. Proteomic Approach to Identify Nuclear Proteins in Wheat Grain. Journal of Proteome Research, 2015, 14 (10), pp.4432-4439. ⟨10.1021/acs.jproteome.5b00446⟩. ⟨hal-01240237⟩