Genome scaffolding with PE-contaminated mate-pair libraries

Kristoffer Sahlin 1 Rayan Chikhi 2, 3 Lars Arvestad. 1
3 BONSAI - Bioinformatics and Sequence Analysis
Université de Lille, Sciences et Technologies, Inria Lille - Nord Europe, CRIStAL - Centre de Recherche en Informatique, Signal et Automatique de Lille - UMR 9189, CNRS - Centre National de la Recherche Scientifique
Abstract : Scaffolding is often an essential step in a genome assembly process,in which contigs are ordered and oriented using read pairs from a combination of paired-ends libraries and longer-range mate-pair libraries. Although a simple idea, scaffolding is unfortunately hard to get right in practice. One source of problem is so-called PE-contamination in mate-pair libraries, in which a non-negligible fraction of the read pairs get the wrong orientation and a much smaller insert size than what is expected. This contamination has been discussed in previous work on integrated scaffolders in end-to-end assemblers such as Allpaths-LG and MaSuRCA but the methods relies on the fact that the orientation is observable, \emph{e.g.}, by finding the junction adapter sequence in the reads. This is not always the case, making orientation and insert size of a read pair stochastic. Furthermore, work on modeling PE-contamination has so far been disregarded in stand-alone scaffolders and the effect that PE-contamination has on scaffolding quality has not been examined before. We have addressed PE-contamination in an update of our scaffolder BESST. We formulate the problem as an Integer Linear Program (ILP) and use characteristics of the problem, such as contig lengths and insert size, to efficiently solve the ILP using a linear amount (with respect to the number of contigs) of Linear Programs. Our results show significant improvement over both integrated and standalone scaffolders. The impact of modeling PE-contamination is quantified by comparison with the previous BESST model. We also show how other scaffolders are vulnerable to PE-contaminated libraries, resulting in increased number of misassemblies, more conservative scaffolding, and inflated assembly sizes. The model is implemented in BESST. Source code and usage instructions are found at https://github.com/ksahlin/BESST. BESST can also be downloaded using PyPI.
Type de document :
Communication dans un congrès
WABI 2015, 2015, Atlanta, United States. WABI 2015, extended abstract, <10.1101/025650>
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https://hal.archives-ouvertes.fr/hal-01236176
Contributeur : Rayan Chikhi <>
Soumis le : mardi 1 décembre 2015 - 13:42:11
Dernière modification le : jeudi 7 janvier 2016 - 16:50:18

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Kristoffer Sahlin, Rayan Chikhi, Lars Arvestad.. Genome scaffolding with PE-contaminated mate-pair libraries. WABI 2015, 2015, Atlanta, United States. WABI 2015, extended abstract, <10.1101/025650>. <hal-01236176>

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