Spatial patterns of bacteria show that members of higher taxa share ecological characteristics

Laurent Philippot (1) , David Bru (1) , Nicolas Saby (2) , Jiri Cuhel (3, 4) , Dominique Arrouays (2) , Miloslav Simek (3, 4) , Sara Hallin (5)

1 MSE - Microbiologie du Sol et de l'Environnement
2 InfoSol - InfoSol
3 Faculty of Science
4 CAS - Czech Academy of Sciences [Prague]
5 Department of Microbiology

Whether bacteria display spatial patterns of distribution and at which level of taxonomic organisation such patterns can be observed are central questions in microbial ecology. Here we investigated how the total and relative abundances of eight bacterial taxa at the phylum or class level were spatially distributed in a pasture by using quantitative PCR. Geostatistical modelling was used to analyse the spatial patterns of the taxa distributions. To test whether the spatial distributions of the different taxa were related to soil heterogeneity, we performed exploratory analyses of relationships between abundance of the bacterial taxa and key soil properties. The distributions of the relative abundance of most taxa varied by a factor of 2.5 to 6.5 and displayed strong spatial patterns at the field scale with autocorrelation ranging between 2 to 37 m. These spatial patterns were taxon-specific and correlated to soil properties, which indicates that members of a bacterial clade defined at high taxonomical levels shared specific ecological traits in the pasture. Overall, the present study showed spatial patterns of distribution of bacteria both at the meter scale and at high taxonomical levels of organisation. Such spatial patterns allow comprehensive observations and predictions of bacterial occurrence in nature, hence helping in the generation of hypotheses concerning the mechanisms generating and maintaining bacterial diversity. The taxa-specific spatial patterns observed here suggest that, in a given environment, ecological traits are shared at high taxonomic levels within the domain Bacteria. This is a piece of evidence that the 16S rRNA gene tree divisions are not only based on evolutionary theory, but also have an ecological reality.

Mots clés: bacteria; spatial pattern; ecology; taxa

Domaines: Sciences du Vivant [q-bio]

Laurent Philippot, David Bru, Nicolas Saby, Jiri Cuhel, Dominique Arrouays, et al.. Spatial patterns of bacteria show that members of higher taxa share ecological characteristics. 10. Symposium on Bacterial Genetics and Ecology - Coexisting on a Changing Planet (BAGECO 10), Jun 2009, Uppsala, Sweden. 1 p., 2009, BAGECO 10 Bacterial Genetics and Ecology - Coexisting on a Changing Planet. ⟨hal-01231305⟩