Development of novel enzyme potentiometric biosensor based on pH-sensitive field-effect transistors for aflatoxin B1 analysis in real samples

K. V. Stepurska (1, 2, 3) , O. O. Soldatkin (1, 2) , V. M. Arkhypova (1) , A.P. Soldatkin (1, 2) , Florence Lagarde (3) , Nicole Jaffrezic-Renault (3) , S. V. Dzyadevych (2, 1)

1 Lab Biomol Elect
2 Institute of High Technologies [Kyiv]
3 ISA-Interfaces & biosensors - Interfaces & biocapteurs

This study aimed at the development and optimization of a potentiometric biosensor based on pH-sensitive field-effect transistors and acetylcholinesterase for aflatoxin B1 determination in real samples. Optimal conditions for bioselective elements operation were defined and analytical characteristics of the proposed biosensor were studied. The proposed biosensor characterized high operational stability and reproducibility of signal. Selectivity of acetylcholinesterase-biosensor to aflatoxins in relation to other groups of toxic substances was analyzed. The developed biosensor was applied to the determination of aflatoxin B1 in real samples (sesame, walnut and pea).

Mots clés: CONDUCTOMETRIC BIOSENSOR; B-1; INHIBITION; MAIZE; GLYCOALKALOIDS; MYCOTOXINS; EXTRACTION; PISTACHIO; ISFETS; Inhibitory analysis; pH-sensitive field-effect transistors; Biosensor; Acetylcholinesterase; Aflatoxin B1; Real samples

Domaines: Chimie analytique; Autre

K. V. Stepurska, O. O. Soldatkin, V. M. Arkhypova, A.P. Soldatkin, Florence Lagarde, et al.. Development of novel enzyme potentiometric biosensor based on pH-sensitive field-effect transistors for aflatoxin B1 analysis in real samples. Talanta, 2015, 144, pp.1079-1084. ⟨10.1016/j.talanta.2015.07.068⟩. ⟨hal-01230636⟩