Electrically addressable deposition of diazonium-functionalized antibodies on boron-doped diamond microcells for the detection of ochratoxin A

Amani Chrouda 1, 2 Amel Sbartai 2 François Bessueille 3 Louis Renaud 4 Abderrazak Maaref 1 Nicole Jaffrezic-Renault 2, *
* Corresponding author
2 Interfaces & biosensors - Interfaces & biocapteurs
ISA - Institut des Sciences Analytiques
3 Surfaces
ISA - Institut des Sciences Analytiques
4 INL - LOCI - INL - Lab-On-Chip et Instrumentation
INL - Institut des Nanotechnologies de Lyon
Abstract : This work reports the manufacturing procedure for an impedimetric immunosensor for sensitive detection of the mycotoxin, ochratoxin A (OTA), through electroaddressing of diazonium functionalized antibodies on the working electrode of a planar Boron Doped Diamond (BDD) electrochemical microcell. The immunosensor elaboration and the immunochemical reaction between ochratoxin A and the surfacebound antibody were monitored using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The impedance variation due to the specific antibody-OTA interaction was correlated with the OTA concentration in the samples. The increase in electron-transfer resistance values presents a sigmoidal shape versus log concentration of OTA, with a dynamic range between 7 pg mL(-1) and 25 ng mL(-1). A limit of detection (LOD) of 7 pg mL(-1) and a IC50 of 1.2 ng mL(-1) were obtained. The immunosensor thus fabricated exhibited high sensitivity, good reproducibility, long-term stability, and was used for the detection of OTA in real coffee samples with a good recovery rate. The reported validated manufacturing procedure is compatible with the production of microarrays for multidetection.
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https://hal.archives-ouvertes.fr/hal-01230062
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Submitted on : Tuesday, November 17, 2015 - 4:34:11 PM
Last modification on : Thursday, February 7, 2019 - 4:56:52 PM

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Amani Chrouda, Amel Sbartai, François Bessueille, Louis Renaud, Abderrazak Maaref, et al.. Electrically addressable deposition of diazonium-functionalized antibodies on boron-doped diamond microcells for the detection of ochratoxin A. Analytical Methods, Royal Society of Chemistry, 2015, 7 (6), pp.2444-2451. ⟨10.1039/c4ay02899f⟩. ⟨hal-01230062⟩

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