Rapid bacterial identification, resistance, virulence and type profiling using selected reaction monitoring mass spectrometry

Yannick Charretier (1) , Olivier Dauwalder (2, 3) , Christine Franceschi (1) , Elodie Degout-Charmette (1) , Gilles Zambardi (1) , Tiphaine Cecchini (1) , Chloé Bardet (1) , Xavier Lacoux (1) , Philippe Dufour (1) , Laurent Veron (1) , Hervé Rostaing (1) , Véronique Lanet (1) , Tanguy Fortin (1) , Corinne Beaulieu (1) , Nadine Perrot (1) , Dominique Dechaume (1) , Sylvie Pons (1) , Victoria Girard (1) , Arnaud Salvador (4) , Géraldine Durand (1) , Frédéric Mallard (1) , Alain Theretz (1) , Patrick Broyer (1) , Sonia Chatellier (1) , Gaspard Gervasi (1) , Marc van Nuenen (5) , Carolyn Ann Roitsch (1) , Alex van Belkum (1) , Jérôme Lemoine (4) , François Vandenesch (2, 3) , Jean-Philippe Charrier (1)

1 Bio-Mérieux [Marcy l'Etoile]
2 StaPath - Pathogénie des Staphylocoques – Staphylococcal Pathogenesis
3 Centre National de Reference des Staphylocoques
4 ISA-ANABIO-MS - Analyse biomoléculaire par spectrométrie de masse - Biological Analysis by Mass Spectrometry
5 Microbiol Unit, BioMerieux Inc

Mass spectrometry (MS) in Selected Reaction Monitoring (SRM) mode is proposed for in-depth characterisation of microorganisms in a multiplexed analysis. Within 60-80 minutes, the SRM method performs microbial identification (I), antibiotic-resistance detection (R), virulence assessment (V) and it provides epidemiological typing information (T). This SRM application is illustrated by the analysis of the human pathogen Staphylococcus aureus, demonstrating its promise for rapid characterisation of bacteria from positive blood cultures of sepsis patients.

Mots clés: BLOOD CULTURES; SYSTEM; STAPHYLOCOCCUS-AUREUS STRAINS; ABSOLUTE QUANTIFICATION; METHICILLIN-RESISTANT; PROTEINS; PROTEOMICS; PEPTIDES; PANTON-VALENTINE LEUKOCIDIN; GRAM-NEGATIVE BACTERIA

Domaines: Biochimie [q-bio.BM]; Chimie analytique

Yannick Charretier, Olivier Dauwalder, Christine Franceschi, Elodie Degout-Charmette, Gilles Zambardi, et al.. Rapid bacterial identification, resistance, virulence and type profiling using selected reaction monitoring mass spectrometry. Scientific Reports, 2015, 5, pp.Article Number: 13944. ⟨10.1038/srep13944⟩. ⟨hal-01229864⟩