IMGT/HighV-QUEST Statistical Significance of IMGT Clonotype (AA) Diversity per Gene for Standardized Comparisons of Next Generation Sequencing Immunoprofiles of Immunoglobulins and T Cell Receptors
Résumé
The adaptive immune responses of humans and of other jawed vertebrate species
(gnasthostomata) are characterized by the B and T cells and their specific antigen recep-
tors, the immunoglobulins (IG) or antibodies and the T cell receptors (TR) (up to 2.10 12 dif-
ferent IG and TR per individual). IMGT, the international ImMunoGeneTics information
system (http://www.imgt.org), was created in 1989 by Marie-Paule Lefranc (Montpellier Uni-
versity and CNRS) to manage the huge and complex diversity of these antigen receptors.
IMGT built on IMGT-ONTOLOGY concepts of identification (keywords), description (labels),
classification (gene and allele nomenclature) and numerotation (IMGT unique numbering),
is at the origin of immunoinformatics, a science at the interface between immunogenetics
and bioinformatics. IMGT/HighV-QUEST, the first web portal, and so far the only one, for
the next generation sequencing (NGS) analysis of IG and TR, is the paradigm for immune
repertoire standardized outputs and immunoprofiles of the adaptive immune responses. It
provides the identification of the variable (V), diversity (D) and joining (J) genes and alleles,
analysis of the V-(D)-J junction and complementarity determining region 3 (CDR3) and the
characterization of the ‘IMGT clonotype (AA)’ (AA for amino acid) diversity and expression.
IMGT/HighV-QUEST compares outputs of different batches, up to one million nucleotide
sequencesfor the statistical module. These high throughput IG and TR repertoire immuno-
profiles are of prime importance in vaccination, cancer, infectious diseases, autoimmunity
and lymphoproliferative disorders, however their comparative statistical analysis still
remains a challenge. We present a standardized statistical procedure to analyze IMGT/
HighV-QUEST outputs for the evaluation of the significance of the IMGT clonotype (AA)
diversity differences in proportions, per gene of a given group, between NGS IG and TR repertoire immunoprofiles. The procedure is generic and suitable for evaluating significance
of the IMGT clonotype (AA) diversity and expression per gene, and for any IG and TR immu-
noprofiles of any species.
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Licence : CC BY - Paternité
Licence : CC BY - Paternité