Single-molecule and super-resolution microscopies of complex organizations for biological applications

Laurent Cognet 1
1 lp2n-04,lp2n-12
LP2N - Laboratoire Photonique, Numérique et Nanosciences
Abstract : The optical microscopy of single molecules has recently been beneficial for many applications, in particular in biology as acknowledged by the Nobel Prize of Chemistry in 2014. It allows a sub-wavelength localization of isolated molecules and subtle probing of their spatio-temporal nano-environments on living cells. It also allows designing innovative strategies to obtain super-resolved optical images i.e. with resolution below the diffraction limit. In a first part, after introducing “standard” single molecule detection strategies currently used in biology, I will present novel developments in the field. In particular, for many single-molecule microscopy applications, more photostable nanoprobes than fluorescent ones are desirable. For this aim, we developed several years ago far-field photothermal methods based on absorption instead of luminescence. Such approaches do not suffer from the inherent photophysical limitations of luminescent objects and allows the ultra-sensitive detection of tiny absorbing individual nano-objects such as gold nanoparticles down to 5 nm in cells or carbon nanotubes. I will present our current efforts to reduce the size of the functional nano-objects and thus obtain single-molecule photothermal nanoprobes for biomolecules found in confined cellular environment (adhesion sites, synapses etc...) The second part of my presentation will be devoted to the presentation of super-resolution microscopy methods. It is indeed crucial to study a large ensemble of molecules on a single cell while keeping the sub-wavelength localization provided by single molecule microscopy. In order to study the dynamical properties of endogenous membrane proteins found at high densities on living cells we developed a new single molecule super-resolution technique, named uPAINT. Interestingly, uPAINT does not require the use of photo-activable dyes allowing easy multi-color super-resolution imaging and single molecule tracking. Different applications of uPAINT will be presented, in particularly the first demonstration of super-resolution imaging of functional receptors in interaction. This last result was obtained combining super-resolution microscopy and single molecule FRET.
Type de document :
Communication dans un congrès
IMAGING A Workshop of the German-French PhD College “Membranes and Membrane Proteins” UFA 0407, Nov 2014, Strasbourg, France
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https://hal.archives-ouvertes.fr/hal-01223500
Contributeur : Laurent Cognet <>
Soumis le : lundi 2 novembre 2015 - 18:25:24
Dernière modification le : jeudi 11 janvier 2018 - 06:26:46

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Laurent Cognet. Single-molecule and super-resolution microscopies of complex organizations for biological applications. IMAGING A Workshop of the German-French PhD College “Membranes and Membrane Proteins” UFA 0407, Nov 2014, Strasbourg, France. 〈hal-01223500〉

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