Phenol soluble modulin alpha alters the cell cycle of eukaryotic cells. - Archive ouverte HAL Accéder directement au contenu
Poster De Conférence Année : 2015

Phenol soluble modulin alpha alters the cell cycle of eukaryotic cells.

Résumé

Staphylococcus aureus (SA) is a Gram-positive bacterium responsible for a wide range of infections in humans and animals. We previously demonstrated that SA USA400 MW2 strain induces a G2/M phase delay in human HeLa cells. This phenomenon was associated with the accumulation of the cyclin-dependent kinase Cdk1/cdc2 and with the accumulation of unphosphorylated histone H3. Additionally we showed that the G2 phase was preferential for staphylococcal internalization and intracellular replication (1).The objectives of the study were -to identify the active substances, which are responsible for the cell cycle alteration, -to understand, how this alteration hijacks defense functions of the host cells.Using size exclusion chromatography of MW2 supernatant, followed by mass spectroscopy analysis, we identified phenol-soluble modulin alpha (PSMα) peptides as the likely candidates for this effect. Indeed, synthetic PSMα1 and PSMα3 caused a G2/M phase transition delay. The implication of PSMα in cell cycle alteration was confirmed by comparison of wild type SA USA300 (strain LAC wt) with the isogenic mutant (LAC∆psmα), lacking the psmα operon that encodes PSMα1 to 4, for its internalization efficiency in HeLa cells. The decreased internalization rate of LAC∆psmα suggested a role of PSMα in host cell invasion. Furthermore, PSMα-induced G2/M-transition delay correlated with a decrease in the defensin genes expression suggesting a diminution of antibacterial functions of epithelial cells (2). Our results open new perspectives for the investigation of the mechanisms of the SA infection.
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Dates et versions

hal-01209855 , version 1 (02-10-2015)

Identifiants

  • HAL Id : hal-01209855 , version 1
  • PRODINRA : 312109

Citer

Martine Deplanche, Rachid El-Aouar Filho, Luidmila Alexseeva, Emilie Ladier, Julien Jardin, et al.. Phenol soluble modulin alpha alters the cell cycle of eukaryotic cells.. 6. Congress of European Microbiologists (FEMS 2015), Jun 2015, Maastricht, Netherlands. , 2015. ⟨hal-01209855⟩
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