O-Glycosylation as a Novel Control Mechanism of Peptidoglycan Hydrolase Activity

Thomas Rolain (1) , Elvis Bernard (2, 1) , Audrey Beaussart (1) , Herve Degand (1) , Pascal Courtin (2) , Wolfgang Egge-Jacobsen (3) , Peter A. Bron (4, 5, 6) , Pierre Morsomme (1) , Michiel Kleerebezem (7, 4, 5) , Marie-Pierre Chapot-Chartier (2) , Yves F. Dufrene (1) , Pascal Hols (1)

1 Institut des Sciences de la Vie
2 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
3 Department of Molecular Biosciences [Oslo]
4 NIZO [Ede, Netherlands]
5 TI Food and Nutrition
6 Kluyver Centre for Genomics of Industrial Fermentations
7 Host Microbe Interactomics Group

Acm2, the major autolysin of Lactobacillus plantarum, is a tripartite protein. Its catalytic domain is surrounded by an O-glycosylated N-terminal region rich in Ala, Ser, and Thr (AST domain), which is of low complexity and unknown function, and a C-terminal region composed of five SH3b peptidoglycan (PG) binding domains. Here, we investigate the contribution of these two accessory domains and of O-glycosylation to Acm2 functionality. We demonstrate that Acm2 is an N-acetylglucosaminidase and identify the pattern of O-glycosylation (21 mono-N-acetylglucosamines) of its AST domain. The O-glycosylation process is species-specific as Acm2 purified from Lactococcus lactis is not glycosylated. We therefore explored the functional role of O-glycosylation by purifying different truncated versions of Acm2 that were either glycosylated or nonglycosylated. We show that SH3b domains are able to bind PG and are responsible for Acm2 targeting to the septum of dividing cells, whereas the AST domain and its O-glycosylation are not involved in this process. Notably, our data reveal that the lack of O-glycosylation of the AST domain significantly increases Acm2 enzymatic activity, whereas removal of SH3b PG binding domains dramatically reduces this activity. Based on this antagonistic role, we propose a model in which access of the Acm2 catalytic domain to its substrate may be hindered by the AST domain where O-glycosylation changes its conformation and/or mediates interdomain interactions. To the best of our knowledge, this is the first time that O-glycosylation is shown to control the activity of a bacterial enzyme.

Mots clés: lactobacillus-plantarum wcfs1; diamino acid endopeptidase; lactococcus-lactis; teichoic-acids; staphylococcus-aureus; bacterial-peptidoglycan; cell-separation; n-acetylglucosaminidase; listeria-monocytogenes; protein glycosylation

Domaines: Autre [q-bio.OT]

Thomas Rolain, Elvis Bernard, Audrey Beaussart, Herve Degand, Pascal Courtin, et al.. O-Glycosylation as a Novel Control Mechanism of Peptidoglycan Hydrolase Activity. Journal of Biological Chemistry, 2013, 288 (31), pp.22233 - 22247. ⟨10.1074/jbc.M113.470716⟩. ⟨hal-01204414⟩