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Article Dans Une Revue Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids Année : 2014

Inhibition of very long acyl chain sphingolipid synthesis modifies membrane dynamics during plant cytokinesis

Résumé

Plant cytokinesis requires intense membrane trafficking and remodeling to form a specific membrane structure, the cell plate that will ultimately separate the daughter cells. The nature and the role of lipids involved in the formation of the cell plate remain unclear. Plant membranes are particularly rich in sphingolipids such as glucosylceramides with long (16 carbons) or very long (24 carbons) acyl chains. We reveal here that inhibition of the synthesis of sphingolipids with very long acyl chains induces defective cell plates with persistent vesicular structures and large gaps. Golgi-derived vesicles carrying material toward the cell plate display longer vesicle-vesicle contact time and their cargos accumulate at the cell plate, suggesting membrane fusion and/or recycling defects. In vitro fusion experiments between artificial vesicles show that glycosphingolipids with very long acyl chains stimulate lipid bilayer fusion. Therefore we propose that the very long acyl chains of sphingolipids are essential structural determinants for vesicle dynamics and membrane fusion during cytokinesis.

Domaines

Biotechnologies
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Dates et versions

hal-01204151 , version 1 (23-09-2015)

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Diana Molino, Elisabeth van Der Giessen, Lionel Gissot, Kian Hematy, Jessica Marion, et al.. Inhibition of very long acyl chain sphingolipid synthesis modifies membrane dynamics during plant cytokinesis. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 2014, 1841 (10), pp.1422-1430. ⟨10.1016/j.bbalip.2014.06.014⟩. ⟨hal-01204151⟩
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