Sixteen cytosolic glutamine synthetase genes identified in the Brassica napus L. genome are differentially regulated depending on nitrogen regimes and leaf senescence

Mathilde Orsel Baldwin (1, 2, 3) , Michaël Moison (3) , Vanessa Clouet (2) , Justine Thomas (2) , Françoise Leprince (2) , Anne-Sophie Canoy (4) , Jérémy Just (5) , Boulos Chalhoub (5) , Celine Masclaux-Daubresse (3)

1 IRHS - Institut de Recherche en Horticulture et Semences
2 IGEPP - Institut de Génétique, Environnement et Protection des Plantes
3 IJPB - Institut Jean-Pierre Bourgin
4 Groupe de Recherche Génomique Amont
5 URGV - Unité de recherche en génomique végétale

A total of 16 BnaGLN1 genes coding for cytosolic glutamine synthetase isoforms (EC 6.3.1.2.) were found in the Brassica napus genome. The total number of BnaGLN1 genes, their phylogenetic relationships, and genetic locations are in agreement with the evolutionary history of Brassica species. Two BnaGLN1.1, two BnaGLN1.2, six BnaGLN1.3, four BnaGLN1.4, and two BnaGLN1.5 genes were found and named according to the standardized nomenclature for the Brassica genus. Gene expression showed conserved responses to nitrogen availability and leaf senescence among the Brassiceae tribe. The BnaGLN1.1 and BnaGLN1.4 families are overexpressed during leaf senescence and in response to nitrogen limitation. The BnaGLN1.2 family is up-regulated under high nitrogen regimes. The members of the BnaGLN1.3 family are not affected by nitrogen availability and are more expressed in stems than in leaves. Expression of the two BnaGLN1.5 genes is almost undetectable in vegetative tissues. Regulations arising from plant interactions with their environment (such as nitrogen resources), final architecture, and therefore sink-source relations in planta, seem to be globally conserved between Arabidopsis and B. napus. Similarities of the coding sequence (CDS) and protein sequences, expression profiles, response to nitrogen availability, and ageing suggest that the roles of the different GLN1 families have been conserved among the Brassiceae tribe. These findings are encouraging the transfer of knowledge from the Arabidopsis model plant to the B. napus crop plant. They are of special interest when considering the role of glutamine synthetase in crop yield and grain quality in maize and wheat.

Mots clés: nitrogen remobilization

Domaines: Sciences du Vivant [q-bio]

Mathilde Orsel Baldwin, Michaël Moison, Vanessa Clouet, Justine Thomas, Françoise Leprince, et al.. Sixteen cytosolic glutamine synthetase genes identified in the Brassica napus L. genome are differentially regulated depending on nitrogen regimes and leaf senescence. Journal of Experimental Botany, 2014, 65 (14), pp.3927-3947. ⟨10.1093/jxb/eru041⟩. ⟨hal-01204074⟩