RAD-seq mapping of spontaneous masculinization in XX doubled haploid rainbow trout lines

René Guyomard (1) , Yann Guiguen (2) , Maria Bernard (1) , Aurélie Charlet (1) , Nicolas Dechamp (1) , Caroline Hervet (1) , Céline Chantry-Darmon (3) , Francine Krieg (1) , Edwige Quillet (1)

1 GABI - Génétique Animale et Biologie Intégrative
2 LPGP - Laboratoire de Physiologie et Génomique des Poissons
3 LABOGENA - Laboratoire d'Analyse Génétique pour les Espèces Animales

Sex in rainbow trout is primarily determined by a genetic male heterogametic system encoded by a master sex-determining gene sdY. However, the occurrence of spontaneous phenotypic males in all-female doubled haploid (DH) gynogenetic populations suggested that multiple sex-influencing loci could exist in trout as found in other species (tilapia, common carp, Atlantic salmon, zebrafish). In order to get further insight in the genetic determinism of this un-expected masculinization, we crossed a DH XX spontaneously sex-reversed male with a normal female. Two F1 females were then reproduced by mitogynogenesis and their DH progeny (n=168) were genotyped using RAD sequencing. A genome scan of 9500 markers was obtained resulting in a linkage map of 2860 cM. Several putative QTL regions have been identified suggesting that more than one mutation could be involved in the observed deviation from the strict XX/XY sex determinism system.

Mots clés: salmonids; linkage map; sex determinism

Domaines: Sciences du Vivant [q-bio]

René Guyomard, Yann Guiguen, Maria Bernard, Aurélie Charlet, Nicolas Dechamp, et al.. RAD-seq mapping of spontaneous masculinization in XX doubled haploid rainbow trout lines. 10. World Congress on Genetics Applied to Livestock Production (WCGALP), Aug 2014, Vancouver, Canada. ⟨hal-01194148⟩