Understanding Early Post-Mortem Biochemical Processes Underlying Meat Color and pH Decline in the Longissimus thoracis Muscle of Young Blond d'Aquitaine Bulls Using Protein Biomarkers.

Mohammed Gagaoua (1, 2) , Claudia Terlouw (1) , Didier Micol (1) , Abdelghani Boudjellal (2) , Jean-François J.-F. Hocquette (1) , Brigitte Picard (1)

1 UMRH - Unité Mixte de Recherche sur les Herbivores - UMR 1213
2 INATAA - Institut de la Nutrition de l'Alimentation et des Technologies Agro-Alimentaire

Many studies on color biochemistry and protein biomarkers were undertaken in post-mortem beef muscles after ≥24 hours. The present study was conducted on Longissimus thoracis muscles of 21 Blond d'Aquitaine young bulls to evaluate the relationships between protein biomarkers present during the early post-mortem and known to be related to tenderness and pH decline and color development. pH values at 45 min, 3 h, and 30 h post-mortem were correlated with three, seven, and six biomarkers, respectively. L*a*b* color coordinates 24 h post-mortem were correlated with nine, five, and eight protein biomarkers, respectively. Regression models included Hsp proteins and explained between 47 and 59% of the variability between individuals in pH and between 47 and 65% of the variability in L*a*b* color coordinates. Proteins correlated with pH and/or color coordinates were involved in apoptosis or had antioxidative or chaperone activities. The main results include the negative correlations between pH45 min, pH3 h, and pHu and Prdx6, which may be explained by the antioxidative and phospholipase activities of this biomarker. Similarly, inducible Hsp70-1A/B and μ-calpain were correlated with L*a*b* coordinates, due to the protective action of Hsp70-1A/B on the proteolytic activities of μ-calpain on structural proteins. Correlations existed further between MDH1, ENO3, and LDH-B and pH decline and color stability probably due to the involvement of these enzymes in the glycolytic pathway and, thus, the energy status of the cell. The present results show that research using protein indicators may increase the understanding of early post-mortem biological mechanisms involved in pH and beef color development.

Mots clés: protein biomarkers; post-mortem; pH; meat color; biological mechanisms; Blond d’Aquitaine; beef

Domaines: Sciences du Vivant [q-bio]

Mohammed Gagaoua, Claudia Terlouw, Didier Micol, Abdelghani Boudjellal, Jean-François J.-F. Hocquette, et al.. Understanding Early Post-Mortem Biochemical Processes Underlying Meat Color and pH Decline in the Longissimus thoracis Muscle of Young Blond d'Aquitaine Bulls Using Protein Biomarkers.. Journal of Agricultural and Food Chemistry, 2015, 63 (30), pp.6799-6809. ⟨10.1021/acs.jafc.5b02615⟩. ⟨hal-01191442⟩