Drought responses in Populus euphratica: effects on water relations, growth, hydraulic properties and gas exchange

Marie-Béatrice Bogeat-Triboulot (1) , Didier Le Thiec (1) , David Hukin (1) , Hervé Cochard (2) , Erwin Dreyer (1)

1 EEF - Ecologie et Ecophysiologie Forestières [devient SILVA en 2018]
2 PIAF - Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier

Populus euphratica species is famous for its ability to cope with high salinity. Moreover, its distribution area comprises deserts with very hot and dry summers (and very cold winters). All species of the Populus genus are know to be drought sensitive and a phreatophytic be-haviour of P. euphratica may explain its ability to grow in such dry areas. In this experiment, we studied the ecophysiological responses of P. euphratica to an increasing drought stress. Plantlets of P. euphratica were obtained by in vitro culture (from Ein Avdat natural park, Israel). After ex vitro acclimation, they were transferred and acclimated to Nancy's green-house. Climatic conditions depended on outside weather but temperature was regulated between 15 and 32°C. A moderate, increasing drought stress was applied and controlled for 6 weeks. Soil volu-metric water content was measured one to two times a day depending on the intensity of the stress (TDR and weighting). Plantlets were progressively brought to 5 stress levels: 10%, 7.5%, 5%, 3% HV and back to fully available water. Although soil water content decreased down to as 3.5%, predawn and minimum water potentials remained above –0.8 and –1.1 MPa, respectively. At this stage, relative leaf water content had declined from 96 to 89% and severe stress symptoms such as leaf yellowing, wilting and shedding were observed. Diameter growth significantly decreased as soon as soil volumetric water content (SWC) reached 16% and then very rapidly stopped. Height growth was affected at SWC around 12%, but reached 0 only when SWC has dropped to 6%. Leaf emission rate was affected 4 days after diameter growth reduction. Stomatal conductance (gs) decreased very early, as soon as soil moisture was less than 10%. Net CO2 assimilation decreased later than gs, only when SWC reached 7-8%. A complete recovery of net CO2 assimilation occurred about 5-6 days after rewatering but stomatal conductance did not completely recover even after 11 days. Water stress had no effect on total chlorophyll content. Soil-to-leaf hydraulic conduc-tance (gL) decreased with drought. Embolism developed early, when drought was rather weak and progressed with increasing stress to 60% PLC. For a given stress level, roots were less embolised than stem. Although P. euphratica is tolerant to salinity, our results demonstrate that it is definitely a drought sensitive species; trees may grow and survive only when the access to a water source such as a water table is possible. This clearly explains why this species is mostly confined to river banks in arid zones.

Mots clés: DROUGHT; POPULUS EUPHRATICA

Domaines: Sciences du Vivant [q-bio]

Marie-Béatrice Bogeat-Triboulot, Didier Le Thiec, David Hukin, Hervé Cochard, Erwin Dreyer. Drought responses in Populus euphratica: effects on water relations, growth, hydraulic properties and gas exchange. Impacts of the Drought and Heat in 2003 on Forests, Nov 2004, Freiburg, Germany. ⟨hal-01191054⟩