A methodology for coupling DGGE and mathematical modelling: application in bio-hydrogen production

Abstract : Molecular biology techniques provide valuable information in terms of microbial dynamic and evolution. DGGE analysis is one of the most popular methods, which has been used in bioprocess assessment. A novel procedure that combines common experimental measurements, DGGE and image analysis is presented as a new methodology for preliminary assessment. In this study, the methodology was applied as an example to the start up of a hydrogen bioreactor, in order to have a preliminary estimation of the actual concentration of the different microbial species. The obtained results are used for determining the kinetic parameter by using a simple mathematical model focusing on species dynamic.
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  • HAL Id : hal-01190372, version 1
  • PRODINRA : 196473

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Estella Tapia, Andres Donoso, Léa Cabrol, Madelena Alves, Alcina Pereira, et al.. A methodology for coupling DGGE and mathematical modelling: application in bio-hydrogen production. 13. IWA World Congress on Anaerobic Digestion, Jun 2013, Santiago de Compostela, Spain. ⟨hal-01190372⟩

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