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A doubly responsive probe for the detection of Cys4-tagged proteins

Abstract : Recombinant proteins bearing a tag are crucial tools for assessing protein location or function. Small tags such as Cys4 tag (tetracysteine; Cys–Cys–X–X–Cys–Cys) are less likely disrupt protein function in the living cell than green fluorescent protein. Herein we report the first example of the design and synthesis of a dual fluorescence and hyperpolarized 129Xe NMR-based sensor of Cys4-tagged proteins. This sensor becomes fluorescent when bound to such Cys4-tagged peptides, and the 129Xe NMR spectrum exhibits a specific signal, characteristic of the biosensor-peptide association.
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Contributor : Serge Palacin <>
Submitted on : Tuesday, November 17, 2015 - 11:06:58 AM
Last modification on : Tuesday, December 15, 2020 - 8:20:04 PM
Long-term archiving on: : Friday, April 28, 2017 - 4:32:06 PM


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N. Kotera, E. Dubost, G. Milanole, E. Doris, E. Gravel, et al.. A doubly responsive probe for the detection of Cys4-tagged proteins. Chemical Communications, Royal Society of Chemistry, 2015, 5, pp.11482-11484. ⟨10.1039/C5CC04721H⟩. ⟨hal-01187782⟩



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