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Peripheral phenotype and gene expression profiles of combined liver-kidney transplant patients

Abstract : BACKGROUND AND AIMS: The beneficial effect of one graft on another has been reported in combined transplantation but the associated mechanisms and biological influence of each graft have not yet been established. METHODS: In multiple analyses, we explored the PBMC phenotype and signature of 45 immune-related messenger RNAs and 754 microRNAs from a total of 235 patients, including combined liver-kidney transplant recipients (CLK), patients with a liver (L-STA) or kidney (K-STA) graft only under classical immunosuppression and patients with tolerated liver (L-TOL) or kidney grafts (K-TOL). RESULTS: CLK show an intermediary phenotype with a higher percentage of peripheral CD19(+) CD24(+) CD38(Low) memory B cells and Helios(+) Treg cells, two features associated with tolerance profiles, compared to L-STA and K-STA (P \textless 0.05, P \textless 0.01). Very few miRNA were significantly differentially expressed in CLK vs. K-STA and even fewer when compared to L-STA (35 and 8, P \textless 0.05). Finally, CLK are predicted to share common miRNA targets with K-TOL and even more with L-TOL (344 and 411, P = 0.005). Altogether CLK display an intermediary phenotype and gene profile, which is closer to that of liver transplant patients, with possible similarities with the profiles of tolerant patients. CONCLUSION: These data suggest that CLK patients show the immunological influence of both allografts with liver having a greater influence
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Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Wednesday, August 26, 2015 - 4:51:09 PM
Last modification on : Tuesday, October 19, 2021 - 11:29:39 AM

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Erwan Dumontet, Richard Danger, Parsia A. Vagefi, Maria-Carlota Londoño, Annaick Pallier, et al.. Peripheral phenotype and gene expression profiles of combined liver-kidney transplant patients. Liver International, Wiley-Blackwell, 2016, 36 (3), pp.401-409. ⟨10.1111/liv.12917⟩. ⟨hal-01187428⟩



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