A novel platform based on immobilized histidine tagged olfactory receptors, for the amperometric detection of an odorant molecule characteristic of boar taint

Abstract : We report a dose-dependent detection of androstenone in solution, as one of the boar taint compounds, based on related OR7D4 olfactory receptors immobilized on a gold electrode through their 6-His tag and NTA-copper complex, as visualized through fluorescence microscopy. Square wave voltammetry (SWV) is for the first time, the method used to monitor the olfactory receptor/odorant recognition process. The relative variation of the Cu(I)-Cu(II) current peak increases linearly versus log (concentration of androstenone) from 10(-14) M to 10(-4) M, in buffer solution. Negative tests were performed, using an unrelated odorant, helional, itself a ligand of OR 1740. Cross-selectivity was also tested after immobilization of OR 1740.
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https://hal.archives-ouvertes.fr/hal-01186656
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Submitted on : Tuesday, August 25, 2015 - 2:05:14 PM
Last modification on : Thursday, February 8, 2018 - 11:10:30 AM

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Zhenzhong Guo, Nadia Zine, Florence Lagarde, Julien Daligault, Marie-Annick Persuy, et al.. A novel platform based on immobilized histidine tagged olfactory receptors, for the amperometric detection of an odorant molecule characteristic of boar taint. Food Chemistry, Elsevier, 2015, 184, pp.1-6. ⟨10.1016/j.foodchem.2015.03.066⟩. ⟨hal-01186656⟩

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