Study and Development of Multilayer Needle-type Enzyme-based Glucose Microsensors

Robert Stemberg (1) , Marie-Bernadette Barrau (1) , Laurent Gangiotti (1) , Daniel R. Thevenot (2, 1) , Dilbir S. Bindra (3) , George S. Wilson (3) , Gilberto Velho (4) , Philippe Froguel (4) , Gérard Reach (4)

1 LABAM - Laboratoire de Bioélectrochimie et Analyse du Milieu
2 CEREVE - Centre d'Enseignement et de Recherche Eau Ville Environnement
3 Department of Chemistry [Kansas]
4 Service de Diabétologie, Hôtel-Dieu de Paris

Glucose oxidase (GOD) was covalently coupled to a cellulose acetate (CA) layer, using bovine serum albumin (BSA) and parabenzoquinone (PBQ) linkages, Prior to GOD coupling this CA layer was deposited on the platinum tip of a needle-type sensor and covered with an outer layer of polyurethane (PU). Such microsensors were found to be active, their GOD load reaching 1.6 to 3.0 pg mm-' and their glucose response reaching I to 3 PA M-' mme2, even when the upper limit of their linear range reached IO-30 mM. Due to the multilayer structure and composition of these micro-sensors, small anions such as ascorbate were partially discriminated from neutral molecules such as hydrogen peroxide. When implanted sub-cutaneously in anaesthetized rats, sensor responses correlated correctly with blood glucose concentration but presented sensitivity coejjicients significantly different to those determined in vitro: a 2 point calibration procedure was found necessary for in vivo experiments.

Mots clés: glucose oxidase; polyurethane; Glucose; microsensor; needle; subcutaneous; cellulose ace-tate; parabenzoquinone

Domaines: Chimie; Chimie analytique; Biochimie [q-bio.BM]

Robert Stemberg, Marie-Bernadette Barrau, Laurent Gangiotti, Daniel R. Thevenot, Dilbir S. Bindra, et al.. Study and Development of Multilayer Needle-type Enzyme-based Glucose Microsensors. Biosensors, 1988, 4, pp.27 - 40. ⟨hal-01179712⟩