TRACE GLUCOSE ELECTRODE FOR CLINICAL, FOOD AND ENVIRONMENTAL DETERMINATIONS

Robert Sternberg (1) , Arnaud Apoteker (1) , Daniel R. Thevenot (1, 2, 3)

1 Laboratoire de Bioêlectrochimie et d'Analyse du Milieu, U.F.R. de Sciences et Technologie, Université Paris—Val de Marne
2 CEREVE - Centre d'Enseignement et de Recherche Eau Ville Environnement
3 LEB - Laboratoire d'Energétique Biochimique

A very sensitive method has been devised for the determination of glucose using electrochemical sensors. The differential device includes a glucose electrode, consisting of a platinum disk covered by a Beta-D-glucose oxidase collagen membrane, and a compensating electrode mounted with a non-enzymatic collagen membrane. Current outputs of both electrodes are substracted and differentiated giving steady-state and dynamic responses proportional to glucose concentration in the 100 nM - 2mM range. The glucose sensor has been successfully tested in clinical (human whole blood, plasma or serum and human seminal plasma), food (fruit, wines, preserved food) and environmental analysis (polluted river, whey) illustrating the high selectivity and versatility of the device.

Domaines: Chimie; Chimie analytique; Biochimie [q-bio.BM]; Sciences de l'environnement; Alimentation et Nutrition

Robert Sternberg, Arnaud Apoteker, Daniel R. Thevenot. TRACE GLUCOSE ELECTRODE FOR CLINICAL, FOOD AND ENVIRONMENTAL DETERMINATIONS. ANALYTICAL CHEMISTRY SYMPOSIA SERIES - vol. 2. Electroanalysis in hygiene, environmental, clinical and pharmaceutical chemistry, The Electroanalytical Group of the Chemical Society, Chelsea College, University of London, Apr 1979, London, United Kingdom. pp.461-473. ⟨hal-01179332⟩