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The (193–209) 17-residues peptide of bovine b-casein is transported through Caco-2 monolayer

Abstract : Although the bioavailability of large peptides with biological activity is of great interest, the intestinal transport has been described for peptides up to only nine residues. b-casein (b-CN,193–209) is a long and hydrophobic peptide composed of 17 amino acid residues (molecular mass 1881 Da) with immunomodulatory activity. The present work examined the transport of the b-CN (193–209) peptide across Caco-2 cell monolayer. In addition, we evaluated the possible routes of the b-CN (193–209) peptide transport, using selective inhibitors of the different routes for peptide transfer through the intestinal barrier. The results showed that the b-CN (193–209) peptide resisted the action of brush-border membrane peptidases, and that it was transported through the Caco-2 cell monolayer. The main route involved in transepithelial transport of the b-CN (193–209) peptide was transcytosis via internalized vesicles, although the paracellular transport via tight-junctions could not be excluded. Our results demonstrated the transport of an intact long-chain bioactive peptide in an in vitro model of intestinal epithelium, as an important step to prove the evidence for bioavailability of this peptide.
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Submitted on : Tuesday, July 7, 2015 - 8:40:06 PM
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Daniela Regazzo, Daniel Mollé, Gianfranco Gabai, Daniel Tomé, Didier Dupont, et al.. The (193–209) 17-residues peptide of bovine b-casein is transported through Caco-2 monolayer. Molecular Nutrition and Food Research, Wiley-VCH Verlag, 2010, 54 (10), pp.1428-1435. ⟨10.1002/mnfr.200900443⟩. ⟨hal-01173380⟩



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