Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR

Abstract : We have developed new methods enabling in vivo localization and identification of metabolites through their (1)H NMR signatures, in a drosophila. Metabolic profiles in localized regions were obtained using HR-MAS Slice Localized Spectroscopy and Chemical Shift Imaging at high magnetic fields. These methods enabled measurement of metabolite contents in anatomic regions of the fly, demonstrated by a decrease in beta-alanine signals in the thorax of flies showing muscle degeneration.
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https://hal.archives-ouvertes.fr/hal-01171918
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Submitted on : Monday, July 6, 2015 - 3:03:33 PM
Last modification on : Wednesday, May 22, 2019 - 4:22:15 PM

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Vincent Sarou-Kanian, Nicolas Joudiou, Fanny Louat, Maxime Yon, Frédéric Szeremeta, et al.. Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR. Scientific Reports, Nature Publishing Group, 2015, 5 (9872), doi:10.1038/srep09872. ⟨10.1038/srep09872⟩. ⟨hal-01171918⟩

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