Anaerobic oxidation of long-chain n-alkanes by the hyperthermophilic sulfate-reducing archaeon, Archaeoglobus fulgidus.

Nadia Khelifi 1 Oulfat Amin Ali 1 Philippe Roche 2 Vincent Grossi 3 Céline Brochier-Armanet 4 Odile Valette 5 Bernard Ollivier 1 Alain Dolla 5 Agnes Hirschler 1, *
* Auteur correspondant
1 MIO - Institut méditerranéen d'océanologie
2 CRCM - Centre de Recherche en Cancérologie de Marseille
3 LGL-TPE - Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement [Lyon]
4 LBBE - Laboratoire de Biométrie et Biologie Evolutive
5 LCB - Laboratoire de chimie bactérienne
Abstract : The thermophilic sulfate-reducing archaeon Archaeoglobus fulgidus strain VC-16 (DSM 4304), which is known to oxidize fatty acids and n-alkenes, was shown to oxidize saturated hydrocarbons (n-alkanes in the range C10-C21) with thiosulfate or sulfate as a terminal electron acceptor. The amount of n-hexadecane degradation observed was in stoichiometric agreement with the theoretically expected amount of thiosulfate reduction. One of the pathways used by anaerobic microorganisms to activate alkanes is addition to fumarate that involves alkylsuccinate synthase as a key enzyme. A search for genes encoding homologous enzymes in A. fulgidus identified the pflD gene (locus-tag AF1449) that was previously annotated as a pyruvate formate lyase. A phylogenetic analysis revealed that this gene is of bacterial origin and was likely acquired by A. fulgidus from a bacterial donor through a horizontal gene transfer. Based on three-dimensional modeling of the corresponding protein and molecular dynamic simulations, we hypothesize an alkylsuccinate synthase activity for this gene product. The pflD gene expression was upregulated during the growth of A. fulgidus on an n-alkane (C16) compared with growth on a fatty acid. Our results suggest that anaerobic alkane degradation in A. fulgidus may involve the gene pflD in alkane activation through addition to fumarate. These findings highlight the possible importance of hydrocarbon oxidation at high temperatures by A. fulgidus in hydrothermal vents and the deep biosphere.The ISME Journal advance online publication, 24 April 2014; doi:10.1038/ismej.2014.58.
Keywords : alkylsuccinate synthase anaerobic hydrocarbon oxidation Archaea hyperthermophilic PflD AF1449
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Article dans une revue
ISME Journal, Nature Publishing Group, 2014, pp.18. 〈10.1038/ismej.2014.58〉
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Soumis le : jeudi 18 juin 2015 - 17:53:31
Dernière modification le : jeudi 20 décembre 2018 - 11:18:46

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Nadia Khelifi, Oulfat Amin Ali, Philippe Roche, Vincent Grossi, Céline Brochier-Armanet, et al.. Anaerobic oxidation of long-chain n-alkanes by the hyperthermophilic sulfate-reducing archaeon, Archaeoglobus fulgidus.. ISME Journal, Nature Publishing Group, 2014, pp.18. 〈10.1038/ismej.2014.58〉. 〈hal-01165315〉

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