Cell Tracking for Assessment of In-Vitro Uptake Kinetics in Ultrasound-Mediated Drug Delivery using Fibered Confocal Fluorescence Microscopy

Marc Derieppe (1) , Baudouin Denis de Senneville (1, 2) , Hugo Kuijf (3) , Clemens Bos (1) , Chrit Moonen (1)

1 University Medical Center [Utrecht]
2 IMB - Institut de Mathématiques de Bordeaux
3 ISI - Image sciences institute - University of Utrecht

Local drug delivery in oncology aims at depositing high doses of anticancer agents while limiting their toxic side effects. Biological barriers, such as cell plasma membranes, hinder their delivery and requires strategies to address this challenge. Previously [1], we demonstrated the feasibility to monitor in real-time, with dynamic fluorescence microscopy, the in-tracellular delivery of a hydrophilic model drug mediated by ultrasound (US), and to quantify the pharmacokinetic parameters derived from a two-compartment model. We evaluate here the impact of the photobleaching (PB) effect experimentally , and compute the PB-corrected uptake kinetics.

Domaines: Traitement du signal et de l'image [eess.SP]

Marc Derieppe, Baudouin Denis de Senneville, Hugo Kuijf, Clemens Bos, Chrit Moonen. Cell Tracking for Assessment of In-Vitro Uptake Kinetics in Ultrasound-Mediated Drug Delivery using Fibered Confocal Fluorescence Microscopy. IEEE International Symposium on Biomedical Imaging (ISBI), Apr 2014, Beijing, China. ⟨hal-01163518v2⟩