A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines

Marine Lambert (1) , Emmanuelle Meudec (1) , Arnaud Verbaere (1) , Gerard Mazerolles (1) , Jérémie Wirth (1) , Gilles Masson (2) , Veronique Cheynier (1) , Nicolas Sommerer (1)

1 SPO - Sciences Pour l'Oenologie
2 Centre de Recherche et d’Expérimentation sur le Vin Rosé

A rapid, sensitive and selective analysis method using Ultra High Performance Liquid Chromatography coupled to triple-quadrupole Mass Spectrometry (UHPLC-QqQ-MS) has been developed for the quantification of polyphenols in rosé wines. The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, the present method allows the selective quantification of up to 152 phenolic and two additional non-phenolic wine compounds in 30 min without sample purification or pre-concentration, even at low concentration levels. This method was repeatably applied to a set of 12 rosé wines and thus proved to be suitable for high-throughput and large-scale metabolomics studies.

Mots clés: Multiple Reaction Monitoring—MRM; UHPLC; polyphenols; rosé wine

Domaines: Sciences agricoles

Marine Lambert, Emmanuelle Meudec, Arnaud Verbaere, Gerard Mazerolles, Jérémie Wirth, et al.. A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines. Molecules, 2015, 20, pp.7890-7914. ⟨10.3390/molecules20057890⟩. ⟨hal-01162586v2⟩