Biomolecules immobilization using the aryl diazonium electrografting

Abdellaoui Sofiene (1) , B. C. Corgier (1) , C. A. Mandon (1) , B. Doumeche (1) , C. A. M. Marquette (1) , L. J Blum (1)

1 ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires

The electrochemical detection of dehydrogenase activity in crude cell lysates is performed simultaneously using 96 carbon electrodes modified with electrografted phenazines. The method is applied to the screening of a library of formate dehydrogenase mutants obtained by directed evolution.

Domaines: Biochimie [q-bio.BM]

Abdellaoui Sofiene, B. C. Corgier, C. A. Mandon, B. Doumeche, C. A. M. Marquette, et al.. Biomolecules immobilization using the aryl diazonium electrografting. Electroanalysis, 2013, 25 (3), pp.671-684. ⟨10.1002/elan.201200334⟩. ⟨hal-00917147⟩