Second harmonic generation from tryptophan-rich short peptides: W(n)K(m) and gramicidin A - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Physical Chemistry B Année : 2014

Second harmonic generation from tryptophan-rich short peptides: W(n)K(m) and gramicidin A

Résumé

We report the first hyperpolarizability of a series of tryptophan-rich short peptides with the respective sequence KWK, KWWK, KWWWK, KWWKWWK, where W and K stand for tryptophan and lysine. The measurements were performed with the technique of hyper-Rayleigh scattering in the bulk of an aqueous Tris buffer solution at a pH of 8.5 and a salt concentration of 150 mM at the non-resonant fundamental wavelength of 784 nm. The first hyperpolarizability of the different peptides follows a simple additive model scaling with the number of tryptophan residues contained in the peptide. However, it appears that the first hyperpolarizability response of a single tryptophan residue in the peptide strongly differs from that of an isolated tryptophan. Hence, it is therefore demonstrated that the local environment of the tryptophan residues within the peptide strongly influences its nonlinear optical response. A comparison with the first hyperpolarizability of the natural peptide gramicidin A measured in trifluoroethanol (TFE) further confirms the key role of the local environment on the first hyperpolarizability of tryptophan residues in peptides.
Fichier non déposé

Dates et versions

hal-01142541 , version 1 (15-04-2015)

Identifiants

Citer

J. Duboisset, G. Matar, F. Besson, D. Ficheux, E. Benichou, et al.. Second harmonic generation from tryptophan-rich short peptides: W(n)K(m) and gramicidin A. Journal of Physical Chemistry B, 2014, 118 (35), pp.10413-10418. ⟨10.1021/jp506416s⟩. ⟨hal-01142541⟩
101 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More