Investigating the performance of in situ quantitative nuclear magnetic resonance analysis and applying the method to determine the distribution of saccharides in various parts of carrot roots (Daucus carota L.)

Elsa Bauchard (1, 2) , Herve This (1)

1 GENIAL - Ingénierie, Procédés, Aliments
2 AliXan Inc

In order to explore the performance of the analytical method called in situ quantitative nuclear magnetic resonance spectroscopy - is q NMR - the distribution of glucose, fructose and sucrose in various parts of a carrot root (Daucus carota L) - primary xylem, secondary xylem, phloem, cortex: top part and lower part - was determined. The influence on the quality of spectra of drying samples before analysis was studied, as well as the influence of the length of strips of tissue used in analysis. Finally samples as small as 240 mm(3) could be studied directly, with minimum prior treatment (only drying), along with deuterated water for locking and a sealed capillary tube containing a solution of 0.5% of the sodium salt of (trimethylsilyl)propionic-2,2,3,3-d4 acid, used both as an internal reference and for quantification. With optimized parameters, the coefficients of variation for measurements were observed to have an average value of 0.038, with a standard deviation of 0.047.

Mots clés: Is q NMR; Daucus carota L; root; sucrose; fructose; glucose; saccharides; NMR; carrot

Domaines: Sciences du Vivant [q-bio]

Elsa Bauchard, Herve This. Investigating the performance of in situ quantitative nuclear magnetic resonance analysis and applying the method to determine the distribution of saccharides in various parts of carrot roots (Daucus carota L.). Talanta, 2015, 131, pp.335-341. ⟨10.1016/j.talanta.2014.07.097⟩. ⟨hal-01123404⟩