Genotoxicity of sulcotrione pesticide and photoproducts on Allium cepa root meristem

Eric Goujon (1) , Chaima Sta (1) , Aurélien Trivella (2) , Pascale Goupil (1) , Claire Richard (2) , Gérard G. Ledoigt (1)

1 PIAF - Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier
2 ICCF - Institut de Chimie de Clermont-Ferrand

Contamination by toxic agents in the environment has become matters of concern to agricultural countries. Sulcotrione, a triketone herbicide used to control dicotyledonous weeds in maize culture is rapidly photolyzed on plant foliage and generate two main photoproducts the xanthene-1,9-dione-3,4-dihydro-6-methylsulfonyl and 2-chloro-4-mesylbenzoic acid (CMBA). The aim of this study was to analyze the potential toxicity of the herbicide and the irradiated herbicide cocktail. Cytotoxicity and genotoxicity of non irradiated and irradiated sulcotrione were investigated in Allium cepa test. The sulcotrione irradiation was monitored under sunlight simulated conditions to reach 50% of phototransformation. Concentrations of sulcotrione in the range 5 x 10(-9)-5 x 10(-5) M were tested. Cytological analysis of root tips cells showed that both non irradiated and irradiated sulcotrione caused a dose-dependent decrease of mitotic index with higher cytotoxicity for the irradiated herbicide which can lead to 24.2% reduction of mitotic index compared to water control. Concomitantly, chromosomal aberrations were observed in A. cepa root meristems. Both non irradiated sulcotrione and irradiated sulcotrione induced a dose-dependent increase of chromosomal abnormalities frequencies to a maximal value of 33.7%. A saturating effect in anomaly frequencies was observed in meristems treated with high concentrations of non irradiated sulcotrione only. These data suggest that photolyzed sulcotrione cocktail have a greater cytotoxicity and genotoxicity than parent molecule and question about the impact of photochemical process on environment. (C) 2014 Elsevier Inc. All rights reserved.

Mots clés: 4-hydroxyphenylpyruvate dioxygenase; vicia-faba; bleaching herbicide; vibrio-fischeri; comet assay; inhibition; toxicity; plant; cells; dna-replication stress; Sulcotrione; Photoproducts; Photolysis; Pesticide; Genotoxicity; Allium cepa

Domaines: Biologie végétale

Eric Goujon, Chaima Sta, Aurélien Trivella, Pascale Goupil, Claire Richard, et al.. Genotoxicity of sulcotrione pesticide and photoproducts on Allium cepa root meristem. Pesticide Biochemistry and Physiology, 2014, 113, pp.47-54. ⟨10.1016/j.pestbp.2014.06.002⟩. ⟨hal-01123041⟩