Influence of sediment composition on PAH toxicity using zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) embryo-larval assays

Abstract : Due to hydrophobic and persistent properties, polycyclic aromatic hydrocarbons (PAHs) have a high capacity to accumulate in sediment. Sediment quality criteria, for the assessment of habitat quality and risk for aquatic life, include understanding the fate and effects of PAHs. In the context of European regulation (REACH and Water Framework Directive), the first objective was to assess the influence of sediment composition on the toxicity of two model PAHs, benzo[a]pyrene and fluoranthene using 10 day-zebrafish embryo-larval assay. This procedure was undertaken with an artificial sediment in order to limit natural sediment variability. A suitable sediment composition might be then validated for zebrafish and proposed in a new OECD guideline for chemicals testing. Second, a comparative study of toxicity responses from this exposure protocol was then performed using another OECD species, the Japanese medaka. The potential toxicity of both PAHs was assessed through lethal (e.g. survival, hatching success) and sublethal endpoints (e.g. abnormalities, PMR and EROD) measured at different developmental stages, adapted to the embryonic development time of both species. Regarding effects observed for both species, a suitable artificial sediment composition for PAH toxicity testing was set at 92.5 % dw silica of 0.2-0.5 mm grain size, 5 % dw kaolin clay without organic matter for zebrafish and 2.5 % dw blond peat in more only for Japanese medaka. PAH bioavailability and toxicity were highly dependent on the fraction of organic matter in sediment and of the Kow coefficients of the tested compounds. The biological responses observed were also dependent of the species under consideration. Japanese medaka embryos appeared more robust than zebrafish embryos for understanding the toxicity of PAHs due to the longer exposure duration and it lower sensitivity of sediment physical properties.
Type de document :
Article dans une revue
Environmental Science and Pollution Research, Springer Verlag, 2014, 21, pp.13703 - 13719. 〈10.1007/s11356-014-3502-7〉
Liste complète des métadonnées

Littérature citée [99 références]  Voir  Masquer  Télécharger

https://hal.archives-ouvertes.fr/hal-01107637
Contributeur : Paco Bustamante <>
Soumis le : mercredi 21 janvier 2015 - 11:34:46
Dernière modification le : mardi 29 mai 2018 - 12:50:54
Document(s) archivé(s) le : mercredi 22 avril 2015 - 10:35:42

Fichier

Perrichon et al. 2014 EPSR.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Prescilla Perrichon, Florane Le Bihanic, Paco Bustamante, Karyn Le Menach, Hélène Budzinski, et al.. Influence of sediment composition on PAH toxicity using zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes) embryo-larval assays. Environmental Science and Pollution Research, Springer Verlag, 2014, 21, pp.13703 - 13719. 〈10.1007/s11356-014-3502-7〉. 〈hal-01107637〉

Partager

Métriques

Consultations de la notice

482

Téléchargements de fichiers

515