Reproducing topography and roughness of osteoconductive materials in a microfluidic device for bone tissue engineering

Abstract : This paper is in the framework of tissue engineering, based on bioreactor for cultivating bone-forming cells on biomimetic materials, such as coral scaffold. In order to optimize such process and to have a better understanding of biophysical and biochemical mechanisms involved in osteoblast proliferation, we develop a thermoplastic microfluidic chip for cell growth studies at the cell scale. The surface porosity (100 μm), roughness (aragonite structure <1 μm) and stiffness (8 GPa) of coral scaffolds, which are commonly used in bone-forming bioreactor, were reproduced in the microfluidic chip. To achieve such features, we developed a specific process: i) we proceed firstly to the fabrication of a master mould in elastomer polydimethylsiloxane (PDMS), reproducing the surface geometric properties of the coral ii) to achieve the desired mechanical stiffness, this elastomeric stamp is then transferred in a thermoplastic material by hot embossing techniques. We used Cyclic Olefin Copolymer (COC) as thermoplastic polymer, with a stiffness of 3GPa and a glass transition temperature of 138°C. The pressure (700kPa), temperature (170°C) and time (12mn) parameters were optimized in order to keep the coral surface geometric properties on the COC polymer. SEM imaging was performed to characterize the surface. Finally, fibroblast cells (NIH3T3-GFP) were cultivated in standard culture medium and seeded on the biomimetic surface. In static flow, we compare cell morphology and adhesion in our microfluidic chip and in coral and obtain a similar behaviour. Such biomimicking microfluidic device, based on moulding process, is a promising tool to study parameters influencing cell growth and proliferation such as shear stress or medium properties in the frame of bone tissue engineering.
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Conference papers
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https://hal.archives-ouvertes.fr/hal-01096932
Contributor : Olivier Francais <>
Submitted on : Thursday, December 18, 2014 - 2:50:01 PM
Last modification on : Tuesday, July 9, 2019 - 10:42:03 AM

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  • HAL Id : hal-01096932, version 1

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Bertrand David, Eric Perrin, Lazaar Mélanie, Chabanon Morgan, Bruno Le Pioufle, et al.. Reproducing topography and roughness of osteoconductive materials in a microfluidic device for bone tissue engineering. 4th European Conference on Microfluidics, Dec 2014, Limerick, Ireland. ⟨hal-01096932⟩

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