Combination of 13 C/ 113 Cd NMR, potentiometry, and voltammetry in characterizing the interactions between Cd and two models of the main components of soil organic matter

Véronique Lenoble (1, 2) , Cédric Garnier (3, 1) , Armand Masion (2) , F Ziarelli (4) , Jean-Marie Garnier (2)

1 PROTEE - Processus de Transfert et d'Echanges dans l'Environnement - EA 3819
2 CEREGE - Centre européen de recherche et d'enseignement des géosciences de l'environnement
3 ISM - Institut des Sciences Moléculaires
4 AMU SPEC - Spectropôle - Aix Marseille Université

This work allowed the characterization of the Cd-binding sites of two compounds taken as models for exudates, the main components of soil organic matter (SOM). The studied compounds were exopolysaccharides (EPS), specifically exudates of roots (polygalacturonic acid) and of soil bacteria (Phytagel). Potentiometric acid– base titrations were performed and fitting of the obtained results indicated the presence of two main classes of acidic sites, defined by their pK a values, for both EPS but of a different nature when comparing the two compounds. The two studied exopolysaccharides presented different acidic/ basic site ratios: 0.15 for Phytagel and 0.76 for polyga-lacturonic acid. Spectroscopic techniques (13 C/ 113 Cd NMR, FTIR) distinguished different Cd surroundings for each of the studied EPS, which is in agreement with the titration results. Furthermore, these analyses indicated the presence of –COOH and –OH groups in various proportions for each exopolysaccharide, which should be linked to their reactiv-ity towards cadmium. Cadmium titrations (voltammetric measurements) also differentiated different binding sites for each compound and allowed the determination of the strength of the Cd-binding site of the EPS. Fitting of the results of such voltammetric measurements was performed using PROSECE (Programme d'Optimisation et de Speci-ation Chimique dans l'Environnement), a software coupling chemical speciation calculation and binding parameter optimization. The fitting, taking into account the Cd 2+ /H +

Domaines: Chimie analytique; Interfaces continentales, environnement; Océan, Atmosphère; Géochimie; Environnement et Société; Milieux et Changements globaux

Véronique Lenoble, Cédric Garnier, Armand Masion, F Ziarelli, Jean-Marie Garnier. Combination of 13 C/ 113 Cd NMR, potentiometry, and voltammetry in characterizing the interactions between Cd and two models of the main components of soil organic matter. Analytical and Bioanalytical Chemistry, 2007, 390, pp.749-757. ⟨10.1007/s00216-007-1678-0⟩. ⟨hal-01096823⟩