A quartz crystal microbalance method to study the terminal functionalization of glycosaminoglycans.

D Thakar (1) , E Migliorini (1) , L Coche-Guerente (1) , Rabia Sadir (2) , Hugues Lortat-Jacob (2) , D Boturyn (1) , O Renaudet , P Labbe (1) , Rp. Richter (1)

1 DCM - Département de Chimie Moléculaire
2 IBS - UMR 5075 - Institut de biologie structurale

We demonstrate the quartz crystal microbalance as a novel method to quantify the reaction yields and stability of the terminal conjugation of chemically complex molecules. Oxime ligation is identified as a facile, broadly applicable method for the reducing-end conjugation of glycosaminoglycans that overcomes the limited stability and yield of popular hydrazone ligation

Domaines: Biochimie, Biologie Moléculaire

D Thakar, E Migliorini, L Coche-Guerente, Rabia Sadir, Hugues Lortat-Jacob, et al.. A quartz crystal microbalance method to study the terminal functionalization of glycosaminoglycans.. Chemical Communications, 2014, 50, pp.15148-15151. ⟨10.1039/c4cc06905f⟩. ⟨hal-01096702⟩