Effect of proteases against biofilms of Staphylococcus aureus and Staphylococcus epidermidis

Pierre-Henri Elchinger (1, 2) , C. Delattre (2) , S. Faure (1) , O. Roy (1) , S. Badel (3) , T. Bernardi (3) , C. Taillefumier (1) , P. Michaud (2)

1 ICCF - Institut de Chimie de Clermont-Ferrand
2 IP - Institut Pascal
3 BioFilm Control

Biofilms play a key role in bacterial resistance against antibacterial agents—an issue that causes multiple problems in medical fields, particularly with Staphylococcus biofilms that colonize medical indwelling devices. The literature reports several anti-biofilm strategies that have been applied in medicine. Disrupting the biofilm formation process creates new sites open to colonization by treatment-generated planktonic bacteria, so efforts have turned to focus on strategies to prevent and control the initial Staphylococci adhesion. Here, we investigated the preventive activities of three commercial proteases (Flavourzyme, Neutrase and Alcalase) against biofilm formation by two Staphylococcus strains. Some proteolytic extracts revealed interesting results with Staphylococcus epidermidis and Staphylococcus aureus aureus biofilms.

Mots clés: anti-biofilm; biofilms; fouling; protease; Staphylococcus aureus; Staphylococcus epidermidis

Domaines: Chimie thérapeutique; Bactériologie

Pierre-Henri Elchinger, C. Delattre, S. Faure, O. Roy, S. Badel, et al.. Effect of proteases against biofilms of Staphylococcus aureus and Staphylococcus epidermidis. Letters in Applied Microbiology, 2014, 59 (5), pp.507-513. ⟨10.1111/lam.12305⟩. ⟨hal-01081362⟩