Super-resolution Microscopy Approaches for Live Cell Imaging

Antoine G. Godin (1) , Brahim Lounis (1) , Laurent Cognet (1)

1 lp2n-04,lp2n-12

By delivering optical images with spatial resolutions below the diffraction limit several super-resolution fluorescence microscopy techniques opened new opportunities to study biological structures with details approaching molecular structure sizes. They have now become methods of choice for imaging proteins and their nano-scale dynamic organizations in live cells. In this mini-review, we describe and compare the main far-field super-resolution approaches that allow studying endogenous or overexpressed proteins in live cells.

Mots clés: (up to 6): super-resolution microscopy; nanoscopy; fluorescence microscopy; live-cell imaging; single molecule detection; localization microscopy Running title: Live cell super-resolution imaging

Domaines: Biophysique [physics.bio-ph]

Antoine G. Godin, Brahim Lounis, Laurent Cognet. Super-resolution Microscopy Approaches for Live Cell Imaging. Biophysical Journal, 2014, 107, pp.1777 - 1784. ⟨10.1016/j.bpj.2014.08.028⟩. ⟨hal-01080729⟩