Laser line scanning for fluorescence reflectance imaging: a phantom study and in vivo validation of the enhancement of contrast and resolution

Frédéric Fantoni (1, 2) , Lionel Hervé (2) , Vincent Poher (2) , Sylvain Gioux (3) , Jerome I. Mars (1) , Jean-Marc Dinten (2)

1 GIPSA-SIGMAPHY - GIPSA - Signal Images Physique
2 CEA-LETI - Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information
3 Department of Medicine

Intraoperative fluorescence imaging in reflectance geometry is an attractive imaging modality to non-invasively monitor fluorescence-targeted tumors. In some situations, this kind of imaging suffers from poor res-olution due to the diffusive nature of photons in tissue. The objective of the proposed technique is to tackle this limitation. It relies on the scanning of the medium with a laser line illumination and the acquisition of images at each position of excitation. The detection scheme proposed takes advantage of the stack of images acquired to enhance the resolution and the contrast of the final image. The experimental protocol is described to fully under-stand why we overpass the classical limits and validate the scheme on tissue-like phantoms and in vivo with a preliminary testing. The results are compared with those obtained with a classical wide-field illumination. pub-lished online Oct. 1, 2014.

Domaines: Traitement du signal et de l'image [eess.SP]

Frédéric Fantoni, Lionel Hervé, Vincent Poher, Sylvain Gioux, Jerome I. Mars, et al.. Laser line scanning for fluorescence reflectance imaging: a phantom study and in vivo validation of the enhancement of contrast and resolution. Journal of Biomedical Optics, 2014, 19 (19), pp.106003. ⟨10.1117/1.JBO.19.10.106003⟩. ⟨hal-01078423⟩