Novel contributions on luminescent apatite-based colloids intended for medical imaging

Abstract : The setup of colloidal hybrid nanosystems based on biomimetic calcium phosphate apatites doped with europium ions has recently raised great interest in the pharmacological community, especially due to their bio-inspired character. This is especially relevant in relation with medical imaging for cancer diagnosis. Questions however remain in relation to a number of applicability aspects, some of which have been examined in this contribution. In a first part of this work, we explored further the luminescence properties of such colloidal nanoparticles. We pointed out, upon excitation of europium, the existence of some non-radiative de-excitation via the vibration of O-H oscillators located at the vicinity of the Eu3þ luminescent centers. The replacement of Eu3þ by Tb3þ ions, less prone to non-radiative de-excitation, was then tested in a preliminary way and can be seen as a promising alternative. In a second part of this work, we inspected the possibility to store these colloids in a dry state while retaining a re-suspension ability preserving the nanometer size of the initial nanoparticles, and we propose a functional protocol involving the addition of glucose prior to freeze-drying. We finally showed for the first time, based on titrations of intracellular Ca2þ and Eu3þ ions, that folic acid-functionalized biomimetic apatite nanoparticles were able to target cancer cells that overexpress folate receptors on their membrane, which we point out here in the case of T-47-D breast carcinoma cells, as opposed to ZR-75-1 cells that do not express folate receptors. This contribution thus opens new exciting perspectives in the field of targeted cancer diagnosis, thus confirming the promise of biomimetic apatites-based colloidal formulations.
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Journal of Biomaterials Applications, SAGE Publications, 2014, vol. 28, pp. 697-707. 〈10.1177/0885328212473510〉
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Ahmed Al-Kattan, Véronique Santran, Pascal Dufour, Jeannette Dexpert-Ghys, Christophe Drouet. Novel contributions on luminescent apatite-based colloids intended for medical imaging. Journal of Biomaterials Applications, SAGE Publications, 2014, vol. 28, pp. 697-707. 〈10.1177/0885328212473510〉. 〈hal-01073123〉

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