Epiplasmins and Epiplasm in Paramecium: The Building of a Submembraneous Cytoskeleton

Anne Aubusson Fleury 1, * Geneviève Bricheux 2 Raghida Damaj 3 Michel Lemullois 1, 4 Gérard Coffe 2 Florence Donnadieu 2 France Koll 5 Bernard Viguès 6 Philippe Bouchard 2
* Corresponding author
2 CMES
LMGE - Laboratoire Microorganismes : Génome et Environnement
3 Cytosquelette
LMGE - Laboratoire Microorganismes : Génome et Environnement
6 IHP
LMGE - Laboratoire Microorganismes : Génome et Environnement
Abstract : In ciliates, basal bodies and associated appendages are bound to a submembrane cytoskeleton. In Paramecium, this cytoskeleton takes the form of a thin dense layer, the epiplasm, segmented into regular territories, the units where basal bodies are inserted. Epiplasmins, the main component of the epiplasm, constitute a large family of 51 proteins distributed in 5 phylogenetic groups, each characterized by a specific molecular design. By GFP-tagging, we analyzed their differential localisation and role in epiplasm building and demonstrated that: 1) The epiplasmins display a low turnover, in agreement with the maintenance of an epiplasm layer throughout the cell cycle; 2) Regionalisation of proteins from different groups allows us to define rim, core, ring and basal body epiplasmins in the interphase cell; 3) Their dynamics allows definition of early and late epiplasmins, detected early versus late in the duplication process of the units. Epiplasmins from each group exhibit a specific combination of properties. Core and rim epiplasmins are required to build a unit; ring and basal body epiplasmins seem more dispensable, suggesting that they are not required for basal body docking. We propose a model of epiplasm unit assembly highlighting its implication in structural heredity in agreement with the evolutionary history of epiplasmins.
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Anne Aubusson Fleury, Geneviève Bricheux, Raghida Damaj, Michel Lemullois, Gérard Coffe, et al.. Epiplasmins and Epiplasm in Paramecium: The Building of a Submembraneous Cytoskeleton. Protist, Elsevier, 2013, 164 (4), pp.451-469. ⟨10.1016/j.protis.2013.04.003⟩. ⟨hal-01071900v2⟩

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