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P-gp expression in brown trout erythrocytes: evidence of a detoxification mechanism in fish erythrocytes.

Abstract : Blood is a site of physiological transport for a great variety of molecules, including xenobiotics. Blood cells in aquatic vertebrates, such as fish, are directly exposed to aquatic pollution. P-gp are ubiquitous "membrane detoxification proteins" implicated in the cellular efflux of various xenobiotics, such as polycyclic aromatic hydrocarbons (PAHs), which may be pollutants. The existence of this P-gp detoxification system inducible by benzo [a] pyrene (BaP), a highly cytotoxic PAH, was investigated in the nucleated erythrocytes of brown trout. Western blot analysis showed the expression of a 140-kDa P-gp in trout erythrocytes. Primary cultures of erythrocytes exposed to increasing concentrations of BaP showed no evidence of cell toxicity. Yet, in the same BaP-treated erythrocytes, P-gp expression increased significantly in a dose-dependent manner. Brown trout P-gp erythrocytes act as membrane defence mechanism against the pollutant, a property that can be exploited for future biomarker development to monitor water quality.
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https://hal.archives-ouvertes.fr/hal-01071814
Contributor : Anne Moné <>
Submitted on : Monday, October 6, 2014 - 4:53:56 PM
Last modification on : Thursday, January 11, 2018 - 6:28:14 AM

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Emeline Valton, Christian Amblard, Ivan Wawrzyniak, Frederique Penault-Llorca, Mahchid Bamdad. P-gp expression in brown trout erythrocytes: evidence of a detoxification mechanism in fish erythrocytes.. Sci Rep, 2013, 3, pp.3422. ⟨10.1038/srep03422⟩. ⟨hal-01071814⟩

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