Chemical Composition of Cyperus rotundus L. Tubers Essential Oil from the South of Tunisia, Antioxidant Potentiality and Antibacterial Activity against Foodborne Pathogens

Ismahen Essaidi; Hayet Ben Haj Koubaier; Ahmed Snoussi; Hervé Casabianca; Mohamed Moncef Chaabouni; Nabiha Bouzouita

doi:10.1080/0972060X.2014.895182

Chemical Composition of Cyperus rotundus L. Tubers Essential Oil from the South of Tunisia, Antioxidant Potentiality and Antibacterial Activity against Foodborne Pathogens

Ismahen Essaidi ^{1, 2} Hayet Ben Haj Koubaier ^{1, 2} Ahmed Snoussi ^{1, 2} Hervé Casabianca ³ Mohamed Moncef Chaabouni ^{1, 2} Nabiha Bouzouita ^{1, 2}

1 Higher Institute of Food Industries of Tunis

2 Lab Chim Organ & Struct Synth & Anal Physiochim

3 PNBS - Produits naturels et biosourcés - Natural & Bio-based Products

ISA - Institut des Sciences Analytiques

Abstract : The chemical composition of Cyperus rotundus L. tubers essential oil from the South of Tunisia was analyzed using GC-FID and GC-MS. Fifty compounds were identified representing 84.6 % of the total oil. A new chemotype was found in Tunisia. This essential oil was characterized by sesquiterpenes abundance (78.3 %) mainly represented by cyperene (15.2 %) and cyperotundone (19.7 %). The essential oil (EO) was further fractionated into three fractions (F1: n-pentane, F2: n-pentane/diethyl ether (95/5) and F3: diethyl ether). The antioxidant activity of the EO and its fractions was tested using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging test and beta-carotene bleaching method. Results showed that the whole EO was more active than the fractions with an effective concentration (EC50) of 0.85 +/- 0.03 mg/mL and an antioxidant activity coefficient (AAC) of 396.0 +/- 2.4. The antimicrobial activity of the EO and its fractions was evaluated using the disc diffusion method against six foodborne pathogens and results showed that Staphylococcus aureus was the most inhibited bacterium with an inhibition diameter of 15.2 +/- 0.8 mm for the whole EO.

Keywords : Cyperaceae chemotypes essential oil sesquiterpenes GC-MS antioxidant activity Antibacterial activity ANTIFUNGAL ACTIVITIES EXTRACTION PLANTS

Document type :

Journal articles

Domain :

Chemical Sciences / Analytical chemistry

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Submitted on : Thursday, October 2, 2014 - 11:54:21 AM
Last modification on : Tuesday, September 18, 2018 - 9:38:32 AM

Chemical Composition of Cyperus rotundus L. Tubers Essential Oil from the South of Tunisia, Antioxidant Potentiality and Antibacterial Activity against Foodborne Pathogens

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Chemical Composition of Cyperus rotundus L. Tubers Essential Oil from the South of Tunisia, Antioxidant Potentiality and Antibacterial Activity against Foodborne Pathogens

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