Detoxification of deuterium labeled isomeric cyclic fatty acid monomers fed to rats
Résumé
Cyclic fatty acid monomers (CFAMs) are mainly formed during frying of edible oils. These fatty acids are mixtures of disubstituted 5 or 6 carbon membered ring structures. Some earlier studies have suggested that some of these molecules could be metabolized and detoxified but so far the detoxification mechanism (s) have not yet been fully elucidated. The objective of the present study was to identify the metabolites resulting from the metabolism and detoxification of CFAMs. For that purpose, the 9- 2 H-10-(6-propyl-2-cyclohexenyl)-dodecenoic acid was synthesized and fed to animals along with a CHOW diet. Biological fluids were collected, analyzed using an untargeted metabolomic approach by UPLC-QToF and compared to those of rats fed with the same diet without CFAM. Identifications of CFAMs metabolites in urine of rats fed CFAM indicate that this molecule may be detoxified following two pathways i) β oxidation cycles and ii) hydroxylation in the α position of the ethylenic bond without chain shortening followed by β oxidation, and elimination mostly by glucuronidation. NO 2 derivatives of the metabolites described above were also identified. Increased excretion of medium chain hydroxyl acylcarnitines in the urine of rats fed with CFAM could indicate changes in fatty acid β-oxidation.