Structural and mutational analysis of human Ad37 and canine adenovirus 2 fiber heads in complex with the D1 domain of coxsackie and adenovirus receptor. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2006

Structural and mutational analysis of human Ad37 and canine adenovirus 2 fiber heads in complex with the D1 domain of coxsackie and adenovirus receptor.

Elena Seiradake
  • Fonction : Auteur
Hugues Lortat-Jacob
  • Fonction : Auteur
  • PersonId : 970500
Olivier Billet
  • Fonction : Auteur
Stephen Cusack

Résumé

Adenovirus fibers from most serotypes bind the D1 domain of coxsackie and adenovirus receptor (CAR), although the binding residues are not strictly conserved. To understand this further, we determined the crystal structures of canine adenovirus serotype 2 (CAV-2) and the human adenovirus serotype 37 (HAd37) in complex with human CAR D1 at 2.3 and 1.5A resolution, respectively. Structure comparison with the HAd12 fiber head-CAR D1 complex showed that the overall topology of the interaction is conserved but that the interfaces differ in number and identity of interacting residues, shape complementarity, and degree of conformational adaptation. Using surface plasmon resonance, we characterized the binding affinity to CAR D1 of wild type and mutant CAV-2 and HAd37 fiber heads. We found that CAV-2 has the highest affinity but fewest direct interactions, with the reverse being true for HAd37. Moreover, we found that conserved interactions can have a minor contribution, whereas serotype-specific interactions can be essential. These results are discussed in the light of virus evolution and design of adenovirus vectors for gene transfer.
Fichier principal
Vignette du fichier
PIIS0021925820705521.pdf (3.37 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-01062657 , version 1 (27-05-2021)

Licence

Paternité

Identifiants

Citer

Elena Seiradake, Hugues Lortat-Jacob, Olivier Billet, Eric J Kremer, Stephen Cusack. Structural and mutational analysis of human Ad37 and canine adenovirus 2 fiber heads in complex with the D1 domain of coxsackie and adenovirus receptor.. Journal of Biological Chemistry, 2006, 281 (44), pp.33704-16. ⟨10.1074/jbc.M605316200⟩. ⟨hal-01062657⟩
200 Consultations
21 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More