Hemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis).

Abstract : Little is known about the immune system of cephalopods, in spite of their many highly derived characters within the molluscan clade, including a vertebrate-like high-pressure closed circulatory system. Further the economic importance of cephalopod fisheries, potential for aquaculture, and use as ecotoxicology models demand a thorough understanding of their immune system. In this study, we present a comprehensive characterization of hemocytes in the common cuttlefish Sepia officinalis. Cytological stainings, electron microscopy- and flow cytometry-observations highlight a single granulocyte population with various densities of eosinophilic granules and unstained vesicles. These hemocytes contain acid phosphatase-, lysozyme- and proPO system enzymes, and have high activity in bead phagocytosis assays. Interestingly, bead pre-incubation in plasma results in time-dependent aggregation perhaps resulting from hemocyanin-coating, and decrease in phagocytosis. This study provides the basis for understanding hemocyte-mediated immunity in the common cuttlefish, and essential background for future studies on cephalopod immunity.
Document type :
Journal articles
Complete list of metadatas

Cited literature [81 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-01062067
Contributor : Charles Le Pabic <>
Submitted on : Tuesday, September 9, 2014 - 10:41:14 AM
Last modification on : Thursday, July 25, 2019 - 2:24:04 PM
Long-term archiving on : Wednesday, December 10, 2014 - 11:20:34 AM

File

FSIM-D-14-00092R1_fv.pdf
Files produced by the author(s)

Identifiers

Citation

Charles Le Pabic, Didier Goux, Maryline Guillamin, Georges Safi, Jean-Marc Lebel, et al.. Hemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis).. Fish and Shellfish Immunology, Elsevier, 2014, 40 (2), pp.362-373. ⟨10.1016/j.fsi.2014.07.020⟩. ⟨hal-01062067⟩

Share

Metrics

Record views

420

Files downloads

825