Application of different zeolites for improvement of the characteristics of a pH-FET biosensor based on immobilized urease

O. O. Soldatkin (1) , I. S. Kucherenko (1, 2) , M. K. Shelyakina (1, 3) , E. Soy (4) , K. Kirdeciler (4) , S. Öztürk (4, 5) , Nicole Jaffrezic-Renault (6) , B. Akata (4, 5) , S. V. Dzyadevych (1, 3) , A. P. Soldatkin (1, 3)

1 Lab Biomol Elect
2 Institute of Biology [Kyiv]
3 Institute of High Technologies [Kyiv]
4 Micro & Nanotechnologies Department
5 Cent Lab
6 SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014)

Different modifications of the zeolites Na+-Beta and LTA were applied for improving the working characteristics of a urea biosensor. The bioselective membrane of the biosensor was based on urease and different zeolites co-immobilized with bovine serum albumin on the surface of a pH-FET. It was shown that the biosensors modified with the zeolites H+-Beta30 and H+-Beta50 are characterized by increased sensitivity to urea. The influence of the zeolite concentration on the sensitivity of the biosensors was studied. The optimal concentration of the zeolites H+-Beta30 and H+-Beta50 in the bioselective membrane was 15%. Different variants of co-immobilization of urease and zeolite H+-Beta30 were studied and the optimal method was selected. Thus, a general conclusion is that the urea biosensor sensitivity can be improved using zeolite H+-Beta30 for urease immobilization in the bioselective membrane.

Mots clés: Biosensors; pH-FET; Urease; Zeolites; MULTIBIOSENSOR; MONOLAYERS

Domaines: Chimie analytique

O. O. Soldatkin, I. S. Kucherenko, M. K. Shelyakina, E. Soy, K. Kirdeciler, et al.. Application of different zeolites for improvement of the characteristics of a pH-FET biosensor based on immobilized urease. Electroanalysis, 2013, 25 (2), pp.468-474. ⟨hal-01006343⟩